Signalling

Part:BBa_K750007

Designed by: Sifan Wang,Ruosang Qiu,Shuqin Hu,Zhao Ma,Yunxin Long   Group: iGEM12_XMU-China   (2012-09-19)

LuxR->LuxPR->GFP

This part contains BBa_K750006, LuxPR and RBS1.0 GFP TT. When arabinose is added, the LuxR protein will be produced. And when this part is ligated with BBa_K750001(or BBa_K750002,BBa_K750003,BBa_K750004,BBa_K750005), the GFP will express because of the influence of quorum sensing system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1080
    Illegal BsaI.rc site found at 1807


[edit]
Categories
Parameters
controlK206000
device_typesignalling
n/aLuxR->LuxPR->GFP
proteinsLuxR