Signalling
Part:BBa_K750007
Designed by: Sifan Wang,Ruosang Qiu,Shuqin Hu,Zhao Ma,Yunxin Long Group: iGEM12_XMU-China (2012-09-19)
LuxR->LuxPR->GFP
This part contains BBa_K750006, LuxPR and RBS1.0 GFP TT. When arabinose is added, the LuxR protein will be produced. And when this part is ligated with BBa_K750001(or BBa_K750002,BBa_K750003,BBa_K750004,BBa_K750005), the GFP will express because of the influence of quorum sensing system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1080
Illegal BsaI.rc site found at 1807
[edit]
Categories
Parameters
control | K206000 |
device_type | signalling |
n/a | LuxR->LuxPR->GFP |
proteins | LuxR |